Dr Mukaida he has become certified OB & Gynecologist, as well as Reproductive Endocrinologist in 1990. During the training period, He studied abroad to the University of Miami, School of Medicine, in the Biochemistry and Molecular Biology Department in 1988-90. Also in order to improve his knowledge and skill for assisted reproductive technology(ART), he became a lab director in a private fertility clinic, the Diamond Institute for Infertility in New Jersey, USA in 1990-1995. He was responsible for several pioneer studies for clinical application of vitrification including the several reports of successful birth of day 2-3 embryos cryopreserved by vitrification with straw, and the blastocysts cryopreserved by ultra-rapid vitrification using cryoloop technique in 1998 and 2000. His original educational video for vitrification was selected to receive the prize in Video: ART Category in 61st ASRM/CFAS meeting. He introduced several technical ideas to improve the performance of vitrification such as artificial shrinkage of blastocelic cavitiy as well as laser assisted hatching, He is one of the editorial board members of the Journal of Reproductive Medicine On line(RBM Online). In present, he runs the private ART center (7-800 OPU a year) in Hiroshima, Japan.